Screening the Responsible Impact of Fourteen Bread Wheat (Triticum aestivum L.) Genotypes against...

Seed germination is regarded one of a plant's initial and most important life stages. As a result, this stage determines the success of growth and crop production. The development of drought tolerant genotypes is one strategy to combat the harmful impacts of deficient water stress on plant productivity. In order to determine the relevant response of studied genotypes with identifying resistant genotypes to high PEG6000 stress, an in vitro experiment was conducted to measure and quantify the negative effects of osmatic PEG6000 stress on seed germination and early seedling growth stage of fourteen wheat genotypes. To accomplish this, the experiment was set up in a split plot arrangement with three replications in a two factorial CRD fashion projection with five levels of PEG6000 factors (s) 0 percent (0 Mpa), 5 percent (-0.3 Mpa), 10 percent (-0.6 Mpa), 15 percent (-0.9 Mpa), and 20 percent (-1.2 Mpa) w/v to create artificial drought stress and assess the progressive effect. The findings are translated into effects in terms of fifteen biological characteristics (germination percentage, germination index, seedling length, relative percentage of seedling length, reduction percentage of seedling length, seedling fresh weight, relative percentage of seedling fresh weight, reduction percentage of seedling fresh weight, seedling dry weight, relative percentage of seedling dry weight, reduction percentage of seedling dry weight, tissue water content, vigour index, tolerance index and coefficient of relative inhibition). For confirmation, the experiment was repeated, and the results were the same. The germination % did not appear to be an effective measure of genotypic variance for PEG6000 stress at the early seedling stage, according to the findings. Furthermore, in all wheat genotypes examined, the degree of PEG6000 stress resistance at the early seedling growth stage did not persist. To differentiate and demonstrate the superiority of PEG6000 stress-tolerant genotypes, the authors used PCA plots and Euclidean distance analyses. Overall, genotypes Gimeza-12, Sakha-94, Katela, and Sids-12 are drought tolerant genotypes, as they demonstrated a higher degree of survival when exposed to high levels of induced PEG6000, whereas the remaining genotypes had an intermediate response. This method can be recommended as a tool for determining the mechanisms to the plant breeder for suitable rapid detection criteria of screening drought tolerant genotypes in a large population incorporate with the reduced cost and labour to initiate breeding programmes to develop better drought tolerant genotypes as best parents for genetic characterization through QTL analysis of mapping populations to initiate breeding programmes to develop better drought tolerant genotypes as best parents for genetic characterization through QTL analysis of mapping populations to initiate breeding programmes to develop better drought tolerant genotypes as best

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