Rapid in vitro Propagation of Physalis angulata: A Valuable Medicinal Plant | Asian Journal of Resea

The influence of growth regulators on in vitro callus induction and plant regeneration was studied using Physalis angulata L. leaf and internodal explants. To generate callus and plant regeneration, two independent experiments were conducted. Leaf segments 0.5mm were cultivated on MS basal medium augmented with (BAP (0.5-4.0 mg/l) + 2, 4-D (0.0-1.5 mg/l) in the first experiment. After 12-16 days, callus initiation was best detected in all media. In MS medium fortified with 2, 4-D 1.0 mg/l and BAP 2.0 mg/l, the highest callus growth in terms of 11.75 percent Yellowish Green Friable Callus (YGFC) was reported. Internodal segments of 0.5 mm were cultivated on MS media containing BAP (0.5-4.0 mg/l) + IAA (0.5-3.0 mg/l) + KIN (1.0 -3.0 mg/l) and 2, 4-D (0.5-3.0 mg/l) in the second experiment. Internodal grown into MS medium containing BAP (2.0 mg/l) + KIN (1.0 mg/l) and IAA (1.0 mg/l) demonstrated the best response of shoot induction, with a mean value of 14 shoots per explant. For root induction, excised shoots were placed in rooting medium containing various concentrations of IBA, IAA, and NAA. On a medium enriched with 0.5-5.0 mg/l of both auxins, the roots were begun and well developed. On the medium containing IBA, a number of roots were generated using all three auxins tested. On MS medium supplemented with 3.0 mg/l IBA, a maximum of 35 roots per shoot were generated in 35 days. The well-rooted plantlets were transplanted to the paper cup to harden, and the well-established plants were moved to the field to acclimate.

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