ISOLATION AND RAPD BASED PCR AMPLIFICATION OF GEMOMIC DNA FROM WHITE BUTTON MUSHROOMS (AGARICUS BISP

Despite the fact that Agaricus bisporus is the world's most widely grown mushroom, contemporary breeding techniques for this commercially important fungus have been largely disregarded. This is due in part to the organism's obstinacy in reproducing and the lack of genetic markers. As a result, we're using PCR to create a set of random markers called RAPDs (random amplified polymorphic DNA). In the germplasm of white button mushrooms, random DNA segment amplified using a simple primer appears to be an effective and very sensitive method of identifying parents (white strains and brown strains), single spore isolates, and hybrids (U-3) in the germplasm.

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