Isolation and Identification of Azospirillium zeae from Acacia tortilis at Riyadh, Saudi Arabia..

Isolation and identification of Azospirillium zeae from Acacia tortilis was carried out in Riyadh, Saudi Arabia. In hostile conditions, beneficial bacteria linked with plant roots have the potential to lessen the burden of utilising costly and dangerous agrochemicals. One of the main obstacles in accomplishing the agronomic application of plant beneficial bacteria is finding unique and appropriate bacteria for promoting plant growth. Several biochemical, morphological, and molecular investigations were used to describe a bacterium isolated from the rhizospheric soil of the legume Acacia tortilis. H1P is a motile bacterium that is negative in biotin media, has no growth in 3 percent NaCl, and is negative for D-ribose but positive for D-glucose. The isolate was identified as an Azospirillum species using 16s rDNA and BLASTn, with a 96.9% similarity to Azospirillum zeae H1P. Phylogenetic analysis using MEGA6 software revealed that the isolate has a common ancestor with Azospirillum zeae before splitting off into its own taxon. It is the first time Azospirillum zeae has been isolated from Acacia tortilis.

Please see the link :- https://globalpresshub.com/index.php/AJORIB/article/view/1109