IN VITRO MULTIPLE SHOOT REGENERATION AND ANALYSIS OF GENETIC FIDELITY OF MENTHA PIPERITA L. | BIO...

Using nodal explants from naturally field grown plants, an effective, quick, and repeatable plant regeneration process for Mentha piperita L. was successfully created. MS (Murashige and Skoog, 1962) media supplemented with varied concentrations of 6-benzyladenine (BA) (0.5-3.0 mg/l), Kinetin (KN) (0.5-3.0 mg/l), and 2-isoPentenyladenine (2-iP) (0.5-3.0 mg/l) was used to culture axillary shoot bud proliferation initiated from nodal explants. On medium containing 1.0 mg/l BA, the maximum number of shoots (4.19 0.03) and average length (3.46 0.16) were induced. After each harvest of freshly produced shoots, the original nodal explant was sub-culturing on shoot multiplication media to establish shoot culture. From a single nodal explant, 20-25 shoots were grown in 3 months. Half-strength MS media supplemented with 1.5 mg/l Indole-3-butyric acid (IBA) and 2% sucrose was used to root shoots. When well-developed whole plantlets were transplanted into plastic pots containing a 1:1 soil and vermiculite combination, 81.13 percent of them survived. RAPD markers were used to assess the hardened plants' genetic integrity. Because genetic homogeneity of in vitro produced plants is also required for the creation of high-quality Mentha piperita L. planting material. This methodology can be utilised for commercial propagation as well as to start genetic improvement investigations in the future.

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