DNA INTEGRITY TEST FOR THE BOAR SPERMATOZOA DURING PRESERVATION IN BTS DILUENT | BIONATURE

Under neutral conditions, the comet assay was used to assess DNA integrity influenced by sperm ageing, which is manifested in DNA double strand break. The current study attempted to examine DNA integrity of boar spermatozoa during liquid storage for 24, 48, and 72 hours at 24°C BTS diluent with ascorbic acid as an antioxidant using a modified neutral comet assay, also known as single cell gel electrophoresis. Comet ejaculates samples were taken at 0 hour and then stored at 24°C for 24, 48, and 72 hours. At 0 hour, the percentage of comets was the lowest, and as time passed, it increased. Individual differences between the boars and breeds were non-existent. According to the findings, there was an increased degree of DNA damage after 72 hours of storage, implying that long-term storage can alter the genomic integrity of swine spermatozoa. As a result, sperm DNA damage screening could be employed as an additional sperm function test with diagnostic value in practise.

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