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Associations between ISSR Markers and Quantitative Traits in Bread Wheat Genotypes | Asian Journal..



Analyses of morphological characteristics and molecular markers are critical techniques for estimating genetic variability between genotypes. For the assessment of genetic diversity among 26 bread wheat genotypes, we combined phenotypic characterisation with the inter-simple sequence repeats (ISSR) approach. There were 43 polymorphic bands among the 87 amplified bands. The percent P ranged from 25 to 76.92 percent, with a 46.97 percent average. Per primer, the average number of polymorphic bands was 4.30. UBC-849 and UBC-833, which belonged to the (GT) and (GA) repeats primer groups, produced polymorphism levels of 62.50 and 76.92 percent, respectively. In the case of di-nucleotide repeat primers, the maximum polymorphism level was clearly obtained. The polymorphism information content (PIC) values in this study ranged from 0.04 to 0.19 (UBC-834), with an average of 0.13. The MI values ranged from 0.12 (UBC-819) to 1.50 (UBC-833), with an average value of 0.62. Two markers (UBC-811930bp and UBC-833600bp) have been found to be associated with stem diameter and grain yield per plant, respectively.


Please see the link :- https://globalpresshub.com/index.php/AJORIB/article/view/829


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