A SIMPLIFIED PROCEDURE FOR ISOLATION OF HIGHLY PURIFIED GENOMIC DNA | BIONATURE

The process for thc extraction of genomic DNA is presented, which includes sodium perchlorate exposure and chloroform extractions, followed by SDS and Pronase E treatments. Within 8 hours, the process can be accomplished. Blood, amniotic fluid, tissue culture, cord blood, and organ tissue can all be employed as sources of genomic DNA, and DNA yields of 250-350 g/ml per 5 ml of blood with OD 260/OD 280 ratios of 1.8 can be routinely obtained with this approach. The extracted DNA shows up on an agarose gel clectrophoresis as a high molecular weight species that can be digested directly with a battery of restriction enzymes. We further show that these DNA preparations may be employed as templates for effective amplification of desired DNA sections using the polymerase chain reaction.

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