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EVALUATION OF ANTI-UROPATHOGENIC BACTERIA ACTIVITY OF ALGERIAN HONEY

The study investigated the antibacterial effect of honey against uropathogenic bacteria isolated from clinical samples (Escherichia coli, Morganella morgani, Enterobacter cloacae, Klebsiella pneumoniae, Serratia fonticola, Proteus mirabilis and Pseudomonas aeruginosa). A total of four natural Algerian honeys (NAHs) were investigated for their antibacterial activity against some uropathogenic isolates. They were analyzed for their color, total phenolic content (TPC) and total flavonoid content (TFC). Two different assays were performed to evaluate the antibacterial potential of the NAH samples: agar-well diffusion and disc diffusion methods. Undiluted and two-fold serial dilutions of NAH (50, 25 and 12.5%) were tested to determine zone of inhibition diameters (ZID). Antibiotic susceptibility profiling were performed according to CA-SFM (Antibiogram committee of French society of microbiology). Results showed that TPC values ranged from 0.682 mg GAE/g for NAH1to 0.510 mg GAE/g for NAH2. TFC ranged from 0.166 mg QE/g (NAH1) to 0.102 mg QE/g (NAH4).The color intensity of the honey samples ranged from 1.10±0.11 to 1.44±0.03 mAU. The diameter of ZDI ranged from 7.5 to 13 mm for Escherichia coli, 8–13 mm for Morganella morgani, 6.8-13.40 mm for Klebsiella pneumoniae, 8-13.4 mm for Enterobacter cloacae, 8-13.5 mm for Serratia fonticola, 7.4-14.5 mm for Proteus mirabilis and 7.6-13.2 for Pseudomonas aeruginosa. The highest activity was induced by NAH3, followed by NAH1, NAH4, and NAH2. The clinical isolates of enterobacteriaceae had a higher resistance profile than other Gram-negative bacteria for most commonly prescribed antibiotics. The overall results of this study indicated that Algerian honey could be used to treat urinary tract infections (UTIs) caused by the tested bacteria.


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