POPULATION cytology III. CYTOMORPHOLOGY AND HPTLC procedure in several MORPHOTYPES OF “INDIAN GINSEN
Abstract
W. somnifera (L.) Dunal. (Solanaceae), extremely valued and one amongst the foremost extensively used flavorer tonic, food in Ayurvedas and thought of as "Indian Ginseng". the target of this study was to research the cytomorphological variations of germplasm from completely different study areas and additionally to search out out the chemical characterization among the chosen morphotypes. Out of of these collected germplasms, 2 cytotypes and two morphotypes were studied. Among these 2 cytotypes diploid (2n=24) and tetraploid (2n=48), the tetraploid cytotype showed extremely abnormal microsporogenesis autoimmune disorder. (Cytomixis, Bridges and Laggards). On the opposite hand within the diploid (2n=24) cytotype, the elaborate cell division course is traditional with equal distribution of chromosomes at A-1, traditional microsporogenesis with high spore fertility. Along with these, 2 morphotypes were additionally known inside the tetraploid cytotype. the key chemical constituents rumored from this plant are with anolides during which with aferin A and with anolide A are most important for polyherbal formulations. so to test the amount of those 2 chemical constituents, a simple, sensitive, and correct high performance skinny layer natural process (HPTLC) methodology has been developed for the estimation of withaferin A and withanolide A in several plant components like leaf, root, stem and fruit of those 2 morphotypes I and II. the higher resolution was achieved by mistreatment mobile section Toluene: ethyl radical acetate: acid (5:5:1). Generally roots are thought-about to be enriched with bioactive with anolides. within the gift study, the most contents of withafer in an exceedingly and with anolide A were determined in leaves that are outstanding supply. this work is an effort to determine a link between cytomorphological variabilities and chemical characterization and to search out out the most effective genotype for the society to satisfy the need of the polyherbal formulations of Ashwagandha in Asian nation and abroad.
See more details